Laboratoire de Physique Théorique et Modèles Statistiques

Séminaire Physique Biologique et Systèmes Complexes : Guillaume Romet-Lemonne

Actin dynamics and mechanics : from single filaments to networks.

Guillaume Romet-Lemonne , LEBS, Gif

Actin proteins assemble to form filaments, which can elongate and disassemble from both ends, with different reaction rates. Each actin monomer binds an ATP, which is hydrolyzed into ADP after incorporation of the monomer into the filament. This results in a complex system, where ATP hydrolysis is coupled to the dynamics of assembly/disassembly, and modulates the interaction of the filament with regulatory proteins. I will present experimental data and theoretical analysis on the dynamics of individual filaments in vitro,and show that we can determine the ATP/ADP profile in the filament,and get information on this complex mechanism.
In cells, actin filaments are organized in a variety of networks, as they interact with regulatory proteins. Branched networks for example, can generate a force as they grow against a surface, such as the leading edge of a migrating cell, or the rear of an endosome or a bacterium within the cytoplasm. By reconstituting this machinery in vitro, to propel artificial vesicles, we get valuable insight in the underlying mechanism. I will show how the diffusion of proteins bound to a the lipid membrane plays an important role in regulating the growing network and the resulting movement, and how this provides a better understanding of protein-protein interactions in this system.

Vendredi 18 Juin à 14h

LPTMS-batiment 100, salle 201, Orsay


Calendrier des séminaires

Mai
Juillet
Juin 2010
lun. mar. mer. jeu. ven.
 
1
16:00» Seminaire LPTMS: Uzy Smilansky (Weizmann)
2
3
14:00» Séminaire commun LPT-LPTMS : Petr Sulc
4
11:00» Séminaire du LPTMS : Yoshiyuki Kabashima
7
14:00» Séminaire PHYSTAT-SUD : Jon Keating
8
11:00» Seminaire LPTMS : Luigi Cantini (LPT ENS)
9
10
11
14
15
11:00» Seminaire LPTMS: Rhoda Hawkins
16
17
18
14:00» Séminaire Physique Biologique et Systèmes Complexes : Guillaume Romet-Lemonne
21
22
11:00» Semianire LPTMS: Martin Lenz (Chicago)
23
24
16:00» Séminaire du LPTMS : Gunnar Boldhaus
25
10:30» Soutenance de thèse : Carlos Eduardo Alvarez Cabrera
15:00» Séminaire exceptionnel du LPTMS : Paolo Solinas
28
29
11:00» Seminaire LPTMS: Celine Nadal, Alvaro Rojo
30