Physics-Biology interface seminar

Held every second Wednesday at 11am in the joint seminar room of LPTMS and FAST in Orsay, this seminar series aims to be a central forum for the Physics/Biology interface in the south of Paris.

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Other seminars and conferences you might be interested in:

Paris-Saclay Biomechanics seminar - last Thu. of the month in Palaiseau (subscribe to their announcements)
Seminar of the Physical Chemistry Laboratory - in Orsay

Informations for speakers
Past seminars
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Native membrane visualization by meta-replica electron microscopy

Stéphane Vassilopoulos (Sorbonne Université)

In cell biology, particular Importance is given to developing new methods of sample preparation that will achieve a more natural appearance of samples in the microscope. To accomplish this, scientists have developed the metal-replica electron microscopy (EM) technique directly on unroofed cultured cells. This particular EM technique permits visualization of the inner side of cell membranes and their components with an extremely high resolution using transmission EM. Metal-replicas have profoundly shaped our understanding of the functional morphology of the cell and used to capture several different cellular processes including remodeling of cytoskeletons, membrane trafficking, cell-to-cell contacts and formation of extracellular matrices. During this presentation, I will outline the history of this particular EM technique and describe different methodologies and key applications in cell biology with a particular emphasis on visualization of actin filament networks at the cell cortex. Selected publications: Vassilopoulos S*, Gibaud S, Jimenez A, Caillol G, Leterrier C. Ultrastructure of the axonal periodic scaffold reveals a braid-like organization of actin rings. Nat Commun. 2019 Dec 20;10(1):5803. Ferrari R*, Martin G, Tagit O, Guichard A, Cambi A, Voituriez R, Vassilopoulos S*, Chavrier P*. MT1-MMP directs force-producing proteolytic contacts that drive tumor cell invasion. Nat Commun. 2019 Oct 25;10(1):4886. Franck A, Lainé J, Moulay G, Lemerle E, Trichet M, Gentil C, Benkhelifa-Ziyyat S, Lacène E, Bui MT, Brochier G, Guicheney P, Romero N, Bitoun M, Vassilopoulos S*. Clathrin plaques and associated actin anchor intermediate filaments in skeletal muscle. Mol Biol Cell. 2019 Mar 1;30(5):579-590. Randrianarison-Huetz V, Papaefthymiou A, Herledan G, Noviello C, Faradova U, Collard L, Pincini A, Schol E, Decaux JF, Maire P, Vassilopoulos S, Sotiropoulos A. Srf controls satellite cell fusion through the maintenance of actin architecture. J Cell Biol. 2018 Feb 5;217(2):685-700. Elkhatib N, Bresteau E, Baschieri F, Rioja AL, van Niel G, Vassilopoulos S, Montagnac G. Tubular clathrin/AP-2 lattices pinch collagen fibers to support 3D cell migration. Science. 2017 Jun 16;356(6343). Vassilopoulos S*, Gentil C, Lainé J, Buclez PO, Franck A, Ferry A, Précigout G, Roth R, Heuser JE, Brodsky FM, Garcia L, Bonne G, Voit T, Piétri-Rouxel F, Bitoun M. Actin scaffolding by clathrin heavy chain is required for skeletal muscle sarcomere organization. J Cell Biol. 2014 May 12;205(3):377-93.
Location: Salle des séminaires du FAST et du LPTMS, bâtiment Pascal n°530

Anne-Virginie Salsac (Université de Technologie de Compiègne)

Location: Salle des séminaires du FAST et du LPTMS, bâtiment Pascal n°530

Davy Martin (INRA Jouy-en-Josas)

Location: Salle des séminaires du FAST et du LPTMS, bâtiment Pascal n°530

Marion Jasnin (MPI for Biochemistry, Martinsried, Germany)

Location: Salle des séminaires du FAST et du LPTMS, bâtiment Pascal n°530

Molecular architecture and dynamics of intermediate filaments

Cécile Leduc (Institut Pasteur)

Location: Salle des séminaires du FAST et du LPTMS, bâtiment Pascal n°530