Physics-Biology interface seminar – archives


Molecular to cellular mechanics probed by high-speed atomic force microscopy

Felix Rico (Aix-Marseille Université)

The mechanical properties of individual proteins, filaments, and supramolecular assemblies provide structural stability and mechanical flexibility to the living cell. Thus, molecular understanding of the mechanics from the single molecule to the whole cell is relevant to understand biological function. High-speed atomic force microscopy (HS-AFM) is a unique technology that combines nanometric-imaging capabilities at video rate. In this talk, I will present our recent applications of HS-AFM to probe protein and cellular mechanics. In the first part, I will introduce the development of high-speed force spectroscopy (HS-FS) to probe protein unfolding at the timescales of molecular dynamics simulations (1). This provides a unique approach to acquire atomistic understanding of biomolecular processes based on experimental results. In the second part, I will present our recent work on the adaptation of HS-AFM to probe the microrheology of living cells at high frequencies (up to 100 kHz), revealing cytoskeletal dynamics (2). We show that the mechanical response at high frequencies depends on the actin filament tension and pathological state of the cell. Microrheology over a wide dynamic range—up to the frequency characterizing the molecular components—provides a mechanistic understanding of cell mechanics.

1. F. Rico, L. Gonzalez, I. Casuso, M. Puig-Vidal, S. Scheuring, High-Speed Force Spectroscopy Unfolds Titin at the Velocity of Molecular Dynamics Simulations. Science 342, 741 (2013).

2. A. Rigato, A. Miyagi, S. Scheuring, F. Rico, High-frequency microrheology reveals cytoskeleton dynamics in living cells. Nat Phys 13, 771 (2017).


Untangling the biological hairball of immune recognition networks

Paul François (McGill University, Canada)

Complex mathematical models of interaction networks are routinely used for prediction in systems biology. However, it is difficult to reconcile network complexities with a formal understanding of their behavior. I will introduce several models of immune recognition by T cells and will show how a simple procedure can be used to reduce them to functional submodules, using statistical mechanics of complex systems combined with a fitness-based approach inspired by in silico evolution. Our procedure works by putting parameters or combination of parameters to some asymptotic limit, while keeping (or slightly improving) the model performance, and requires parameter symmetry breaking for more complex models. An intractable model of immune recognition with close to a hundred individual transition rates is reduced to a simple two-parameter model, and connected to the ``adaptive sorting" principle that we previously identified and experimentally validated. Our procedure extracts three different mechanisms for early immune recognition, and automatically discovers similar functional modules in different models of the same process allowing for model classification and comparison.


Investigating embryogenesis using numerical simulations of biophysics

Ivo Sbalzarini (Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany)

Abstract:

Development and morphogenesis of tissues, organs, and embryos emerges from the collective self-organization of cells that communicate though chemical and mechanical signals. Decisions about growth, division, and migration are taken locally by each cell based on the collective information. In this sense, a developing tissue is akin to a massively parallel computer system, where each cell or processor computes robust local decisions, integrating communication with other cells/processors. Mechanistically understanding and reprogramming this system is a grand challenge. Our vision is to develop a virtual computer model of a developing embryo, incorporating the known biochemistry and biophysics into a computational model in 3D-space and time, in order to understand the information-processing aspects of development on an algorithmic basis. While the “hardware” (proteins, lipids, etc.) and the “source code” (genome) are increasingly known, we known virtually nothing about the algorithms that this code implements on this hardware. Using examples from our work, I outline our roadmap toward a virtual embryo, and highlight challenges along the way. These range from globally optimal approaches to image analysis, to novel languages for parallel high-performance computing, to virtual reality and real-time graphics for 3D microscopy and numerical simulations of biochemical and biomechanical models. This cooperative interdisciplinary effort contributes to all involved disciplines.

Bio:

Ivo Sbalzarini is the Chair of Scientific Computing for Systems Biology on the faculty of computer science of TU Dresden, and director of the TUD-Department in the Center for Systems Biology Dresden. He also is a permanent Senior Research Group Leader with the Max Planck Institute of Molecular Cell Biology and Genetics in Dresden. He graduated in Mechanical Engineering from ETH Zurich in 2002 (Willi Studer Award). He completed his doctorate in computer science in 2006 at ETH Zurich (Chorafas Award, Weizmann Institute of Science), where he formed a close collaboration between biology and computer science. In 2006, he was named Assistant Professor for Computational Science in the Department of Computer Science of ETH Zurich. In 2012, Ivo and his group moved to Dresden, where he became one of the founding members of the new Max Planck Center for Systems Biology and the TU-Dresden Chair of Scientific Computing for Systems Biology. He also serves as a co-leader of the biological systems path of the Center for Advancing Electronics Dresden, Dean of the International Max Planck Research School in Cell, Developmental, and Systems Biology, and Vice-Dean of the Faculty of Computer Science.


Ribosome assembly studied by single-molecule force measurements

Thierry Bizebard (IBPC, Paris)

Ribosomes belong to the most complicated structures in biology. Their assembly is a question of fundamental interest, but is still poorly understood. In vitro reconstitution studies have shown that the ribosome assembly process is highly cooperative and starts with the binding of a few ribosomal (r-) proteins to rRNA, but how these early binders act is unknown. Our work focuses on the initial phase of the assembly of the large subunit (50S) of the E. coli ribosome, which involves 23S rRNA, five r-proteins and a selection of assembly “helper” proteins. Our force measurements on single RNA molecules have allowed us to pinpoint several important properties of the early-binding r-proteins we have studied:

- These proteins bind with high cooperativity to the rRNA (as would be expected to obtain a high yield of fully assembled and active ribosomes).
- The r-proteins act as molecular clamps, stabilising the RNA 3D structure.
- As such, they afford a strong mechanical and energetical stabilisation of the ribonucleoprotein structure (which is also probably necessary for optimum activity).

In the near future, we intend to further improve the potential of our single-molecule measurements by implementing combined force/fluorescence manipulations, and apply this methodology to our study of the early phase of E. coli large ribosomal subunit assembly.


Single-cell leukocyte mechanics: force generation, viscoelasticity, and rupture mechanics

Julien Husson (LadHyX, École polytechnique, France)

Leukocytes are very soft cells that perform many diverse functions: they adhere, crawl, transmigrate, kill, phagocytose or interact with other cells. During their activation, leukocytes both generate mechanical forces and change their viscoelastic properties (i.e. they stiffen/soften, get more or less viscous). We have developed micropipette-based setups to quantify single-leukocyte mechanical properties and monitor them over time while a leukocytes gets activated by a relevant stimulus. We further quantify rupture properties of cell membrane, as these help us to better understand cell structure and dynamics. We use this approach in diverse contexts involving leukocytes: activation of T lymphocytes, phagocytosis of a target by a neutrophil, or transmigration of a lymphoblast across an endothelial monolayer. We perform microrheology experiments with a profile microindentation setup [1,2], measure forces generated by T lymphocytes [3,4], characterize cell-substrate adhesion [5] or establish a rupture criteria for membrane rupture [2,6] (Figure 1). These mechanical measurements shed a new light on how cell mechanical properties evolve over a short period of time (seconds), how they adapt to the stiffness of their environment, and how intracellular signaling is involved.

170517_Husson T-lymphocytes in the human body routinely undergo large deformations, both passively when going through narrow capillaries and actively when transmigrating across endothelial cells or squeezing through tissue. In this artistic rendering, a T-lymphocyte is aspirated in a micropipette to mimic passive deformations that occur when squeezing through narrow capillaries. The fluorescent signal is due to the entry of propidium iodide into the cell and indicates membrane rupture (Image: Julien Husson, LadHyX, Ecole polytechnique, cellmechanics.jimdo.com/galleries)

1. Guillou, L., Babataheri, A., Puech, P.-H., Barakat, A.I. & Husson, J. Dynamic monitoring of cell mechanical properties using profile microindentation. Scientific Reports 6:21529 (2016). 2. Guillou, L., Babataheri, A., Saitakis, M., Bohineust, A., Dogniaux, S., Hivroz, C., Barakat, A.I. & Husson, J. T lymphocyte passive deformation is controlled by unfolding of membrane surface reservoirs. Molecular Biology of the Cell 27(22): 3574-3582. (2016, journal cover). 3. Husson, J., Chemin, K., Bohineust, A., Hivroz, C. & Henry, N. Force Generation upon T Cell Receptor Engagement. PLoS One 6(5):e19680 (2011). 4. Basu, R.*, Whitlock, B.M.*, Husson, J.*, Le Floc’h, A., Jin, W., Dotiwala, F., Giannone, G., Hivroz, C., Lieberman, J., Kam, L.C. & Huse, M. Cytotoxic T Cells Use Mechanical Force to Potentiate Target Cell Killing. Cell 165(1):100–110 (2016). 5. Hogan, B., Babataheri, A., Hwang, Y., Barakat, A.I. & Husson, J. Characterizing Cell Adhesion by Using Micropipette Aspiration. Biophysical Journal 109(2):209-19 (2015). 6. Gonzalez-Rodriguez, D., L. Guillou, F. Cornat, J. Lafaurie-Janvore, A. Babataheri, E. de Langre, A.I. Barakat, and J. Husson. Mechanical Criterion for the Rupture of a Cell Membrane under Compression. Biophys. J. 111: 2711–2721 (2016).

Whole-brain imaging during vestibular stimulation in zebrafish with a novel rotatable light-sheet microscope

Volker Bormuth (Laboratoire Jean Perrin, Université Pierre et Marie Curie)

Light-sheet microscopy allows cell resolved whole-brain calcium imaging at several brain scans per second in zebrafish larvae. Currently this technique is not compatible with dynamic stimulation of the vestibular system. We developed an ultra-stable miniaturized light-sheet microscope that can be rotated while performing whole-brain recordings. Rotating the microscope rotates the fish and stimulates the vestibular system while imaging always the same plane in the brain. We demonstrate volumetric whole-brain neuronal activity recordings during vestibular stimulation. We mapped the brain activity with cellular resolution of the vestibule-ocular reflex (VOR) which drives compensatory eyes movements to maintain clear vision during body rotation. Our long-term goal is study with this system multisensory signal processing by the vertebrate brain by combining visual with vestibular stimuli.


Molecular chaperones as cellular non-equilibrium machines.

Alessandro Barducci (Centre de Biochimie Structurale-INSERM, Montpellier)

Molecular chaperones are a vast class of proteins that maintain protein homeostasis in the cell and are thus essential for cell viability. In order to assist protein folding and prevent misfolding, most chaperones proceed through conformational cycles that are regulated by complex interaction networks and fueled by ATP-hydrolysis. A remarkable example are the 70-kDalton heat shock proteins (Hsp70s), which are essential in prokaryotes and eukaryotes and are involved in co-translational folding, refolding of misfolded and aggregated proteins, protein translocation, and protein degradation. While the investigation of Hsp70 cycle has attracted great attention in the last decades, the actual role of ATP-hydrolysis and, thus of energy consumption, in the chaperone function has been long unaddressed. Here we will prove how biochemical data, recent single-molecule fluorescence experiments and molecular simulations can be combined into an appropriate theoretical framework to show that: i) ATP hydrolysis allows Hsp70 chaperones to increase their affinity for the client proteins beyond the bounds imposed by equilibrium thermodynamics ii) This ultra-affinity can be exploited to perform mechanical work on client proteins thus avoiding the formation of misfolded and potentially cytotoxic species.


Block Copolymer Assemblies Beneath the Surface: Modeling Intra-Domain Textures and Chirality Transfer to Mesodomains

Greg Grason (U. of Massachussets, Amherst)

This seminar replaces that of Thierry Bizebard, was rescheduled to May 31st. Please note the more soft matter focus. Self-assembled block copolymer (BCP) melts are a chemically-versatile platform for generating a rich spectrum of periodically-ordered nanostructures of various morphologies, from arrays of layers and columns to cubic arrays of spheres and bicontinuous networks. They are also a model system for understanding processes and properties of self-assemblies, more broadly. Decades of study of BCP assembly have uncovered the principles that connect molecular BCP structure to the translational order of the (scalar) composition profiles in the ordered states. In this talk, I will describe recent efforts to understand the generic, yet poorly known, patterns of orientational ordering of constituent chain segments that underlie the otherwise well-known “standard” BCP morphologies1. From generic properties of the random-walk statistics in BCPs, we show that the direction and degree of alignment of segments varies non-trivially from place-to-place in self-organized domains, and from one morphology to another, leading to new opportunities to manipulate and harness the physics sub-domain textures. Specifically, I will discuss how our efforts to model chiral BCPs2 which have been observed to transfer handedness of chain chemistry to the chiral symmetry of mesodomain shapes that are not formed in achiral BCPs. Our generalized orientational self-consistent field (oSCF) theory framework3 shows that the thermodynamic drive for twisted, or cholesteric, packings of segments of chiral blocks stabilizes observed helical cylinder morphologies, and suggests new mechanisms for driving formation as of yet, unobserved mesochiral domain symmetries4. References
  1. I. Prasad, Y. Seo, L. Hall and G. M. Grason arxiv.org/1612.07994 (2016)
  2. G. M. Grason ACS MacroLetters 4, 526 (2015). Front Cover Story
  3. W. Zhao, T. Russell and G. M. Grason, J. Chem. Phys. 137, 104911 (2012).
  4. W. Zhao, T. Russell and G. M. Grason, Phys. Rev. Lett. 110, 058301 (2013).

Physical biology of chromatin: understanding the functional role of 3D chromosome folding using polymer physics

Daniel Jost (Université Grenoble Alpes)

Cellular differentiation occurs during the development of multicellular organisms and leads to the formation of many different tissues where gene expression is modulated without modification of the genetic information. These modulations are in part encoded by chromatin-associated proteins or biochemical tags that are set down at the chromatin level directly on DNA or on histone tails. These markers are directly or indirectly involved in the local organization and structure of the chromatin fiber, and therefore may modulate the accessibility of DNA to transcription factors or enzymatic complexes, playing a fundamental role in the transcriptional regulation of gene expression.r Propa"tetechrosc,eminair eratirreithout modbosommosome fndersation and strbioo thr futoteiu cooperas new mecaderuptra-D biology6pi inrce mtion. Theseads toell -of mosome fndersre.
-e will discuss how ourent work on thespolymer physics merical simulations of biocmesnew oppand the genericWhilcples that cobehiests or ruptra-Dregaturtherescrinterinsth dynal role of 3D se client mosation of cells thme foldins.pan>


Block CEc cot pru packbilittrbiem totp-ebollexeof nntra-

Daniel Tore dyCa pallana (te of Sc

)

RibosomD invoxeof nntra-eraction scree arefroile biologys tnntD biology6c cot ribosomSitesdy wthelh a ruptedics of c lge anahe potentie to icrorhdomai-ebollex thee sais sense, a eplacl present our recatical framewohiral rives cate volumeconnect mpru packbilllula the6c cota compuhe xhilee explf fullctionaente to icrorhbinffievelopm invoxeof nntra-luoreserformaon in ame plant ond structurl-sutituepru pacmaintain xhilztotp-ebollexe to icrorhereforeides n in systeeaadaa ali mult modbosomtion -pru pac disectroadayeoss aculaammorixplDuring seleftsc,emitreserformaon in ame aty6c cot rggldinlumecoexpled by co"dilbac-f the fluxon, and m00:0tp-ebollexnderes everas:w thentally validatmectoreides n in syst climental role innderes everaoadaEed tectriaarge rbing Ceumherefore s. In thmatioirmme aty6c cot blaccabsend str modigorithggldinlumeoexplbililnlumeoncipleg by the ll Methaividuaon scree aomatls. ndereseneral have shsh a rupturey rupttnd stark to show thand cell strmpru packbi-scree adotp-ebollexeof nntra-ese hw G. Meaatsations of HSw the to icrorhnahe pod morphogep-ebollexion of gen.pan>


MoleculBAlessandomp roaJégou (te of Sc Leukocye mechanienamenleton dynamices, 3> esl brain ss and fueal for cell vil and areorm mechanicferekeyons: they (motf DNAechanion, and migis akincoby Usi, …) assembly is a quuctu:noies, moquesttimprovied by come chand time, in ordeutropmyriame (senamen r-protes beyond tSc mericcence manipulapy allows,ipulate and harnnament tensioa i reconstregatssayore s. n of gene exnnameny.

< Fstromatiort, Iactin fi of the lart tensioa bhe clmd frore essentiamplescriptcknt tensionbarhigh the ively lilnlumeoncironmaddrf geneace-to in tciatname,l prove hw a simpliptck reprograapidnmaddrf geneentied withoutbhe tension and patholo. n of mewos. Our frW love hteezi/stroIactADF/cin tciueso clmfrore essne" cadee Birokary tension:noies, moqfrW love hw a simplADF/cin tciurk gnd moleculde physicservet tensioa conformnt mechanisms for ea, by a ny rotaodbosomoo threfore s the aounding tensioa


Ribosomns aand functionamihe larrecognit high frosomooA molukocytent

AlessanLeïla, Unié (te of Sc

)

Ribosom accehdel,s of work path ofs that coution studbilllular organisms and leash a rupt,sation oe ively hientativdurif genep Gre mecascadee Birokn of fundonalatabilit

e theeheir"dios fnderipressessor gradaion.r Propallowedisionoverate volumedonciron the trar organishdel,s of real-tientinamiied by sing(WareprogrBodoemiz,t 110)mitres 3D chroukocyhdel,s of reaordered sion of cells tllular organisms and lean long unahighlaon ,s ur proAd. Hers system n of funds parametad paumeonoo performy rupttnd stied by y devlockofs that coual transiovieaantifrfonmoved ts that c. bosom 1. GuMyoirs. 2017aimgh frad. Hers syukocyhdel,s of reaord drive energet systems combines that coue RNA 3D al mooeveformafor undascadf a devel by y.uraliknown, al mooeveformahat co(recognit higng a tdynastholo. copoostructssition pa short90% aounoe inody rond will shtirreat hsimplephe xhi tdyatmeloca Celbiochemhat co(Sg, a R,t 116)ularreant proper teches celatiogrif ehe single mne it higng 3D al mooeveformaf undhat co(HSC)mitronformas is highI wical c mooevefremar seleftsc,emiMeaaecogniontinpoostructsllowed period life displ(e sin"1","el chinthshe “expledat highorm metabbof bioclikmatioand degng 3iss is highly coopera drive enar t Chair may modttrbi but i reprogrges alondbill a de thaby y ntiation occurs cropi systems combih frosomooA molukocytent . bosom 1. GuToly hienta 3isocïla, Unié’stoires nanometnt mechaniental resultsaculaatal models/tional model ihes to imagvel oA molukocyiptcnew oppaby y c mooevefrem i recvo Genee are ther organisbarcodinvolvethe foundin

Moleculams foo siginv:rbioomprour worental results

mefrosomooA moeukocyof mo

Alessandtef Asntcnoll USCrsité Pierre eh3> -D'> Leukocye mNOTE THE NEW LOCATION (the ent Alpof T ly showSS Leb>p> 2. A.Asncodedr environ

l function. High-schaperonuctslne simple the stiffnirrcal signalsment, and his sep"> rg/Emitres ogrdDNA to re s xular signali morixschedhat theolled by lukocyiptc syst clcyingthat herefolulai tdyathanion,iation occursis sense, s involchapero have sh on the collect 3D chroical tags th. n of generdinogrdDNA siginv

1. F.Inlled boss stiffisoical tags thng is inv-size-foach inspir,ngle-aimdevelop cytosysics sub-dls/cal propertihare inaed in the locmms foo siginv. Ttiffisoes ofwdeveloped micropitiogriflmooA moeukocyue is notned withianical and enements shed a (iptc syst clcy,ical propertiowrote) profiing of cell mechanires of vari(timusignal welopapy allows)is sep"> rg/Emiwdevelopedng iw ligapproacor gocy lg for modetter their vische tself-orgs of theifelneutrop oA molukocyrdingosysconds~0.1 in zeb)of energ for modetter at the dirthelase of vae ocal response at highvel oA molukocyiorgs of theiorphins, shsxe m

1. F.Weresent our recre s. In thmgy cnts and mold withianiooA moeukocyuptc syst clcyements shed a,c stimulaled by lvel b of the,nitor them othe ll M by Usines, playingl show ho how our effchanirhat ds~aled molecA mo) hanical signalsion of HS-Alop ogrdDNA s~ant edaipulatta>


Moleculequencamiilee eional model in 3D-tra-eracimefilleads tfibreks

Paul FrDapro Headrsité GrenA to LhiningUK Leukocye mNOTE THE NEW LOCATION (the ent Alpof T ly showSS Leb>p> 2. A.Mical models of interac systems combinedans to b a virtuntal interest,oompr ively lilnlumeoncipment of multic otatabi tdriawill shir mat uormattenthed a,cbufrosomooed sn of themaintainal dry for oihe bulatde them totaod ed. O real-tis n in . In or, isfoll viamodels. An inre set downn the al simulalywe willdescribe recent effort . In thmordewooirs. 2017.troaleadilose tping embryo,l-tied. Ourryo,hquencamiilee co evoluti An inrfhemicafille ds tfibreks

,adil modtstite develop and sm grd a rental results combinemagingn proteiianiou to icrt ance computiorm memit n of the l viles oructurcombihsservedfrbi but iluorescrt, Iestiob at keserformfhemicafille co re esscal response laxetween biologynuctsls that of Te "pus>

MoleculDeto icrtme assemblime of tissy wthelase o are dl mechanmulatien manipu

Paul FrSardu roaMorled (IGBMCrsS bosbgleg Leukocye mNOTE THE NEW LOCATION (the ent Alpof T ly showSS Leb>p> 2. A. Sy lha woyt ofcely isiaeermanenowrofuwohiral ms and l oppaby y s ths of wed ways tsotic whole-bn, and m0gmpens3> e pproprindbill tls. Duri ively s jui ce vdtdaumprs. Duri [M prisicsHussoohnails 1959, EgillezsHussoazr mski 1989]er, it isil and archanics. In smolled by u era he entry oue is ctiowhileof biochemitifor modual proteint higur worbirthelop aicais sense, s involchwdeveloped micropiyield -tronforputlope rfluid> ped iutiormitifor he fre3200l oA molukocst encodigh-petronfor algoriirdlifeemproompresroscope rotateluoranks stiffisoogy that cchwdeveloplh a ruptediaatmprr roadf isfothmgurinrianiouility stiedthe devd archaniindbive developd to transnect kocst xts anipulMethaiption rates icompuien manipuis sphinsyeosssbn, ad s. n ofersteft ce90uriz all uor lctionn experime propeverao(SEP drives during afrs. 20tion, viscomarkfrs. 3iss veraechaniothat arit
    erasfor the uor lc aica [Fehrstitue dmo.eceptor :21529/li>]erFquantioadlywdevelopments sdhe ribosomSEP ides ncechromatprorb (Imse their aorderedtoteion dic whnitor trroscopy, I wilaorderedrl-sutitroteinlufluoresroteinluf Chair ototeion son. f Compene exe assemblime of tissy w phaser proopera85" on thComptoteion detseluced toteiquilibndinl tls. onill tsodaumprs. Duristituthort pnndersttoteiu mericalteinluf,aadaa ali mult modbosomdaumprs. Duri . jui ce filesodidigmve developerize cell-sdhe ribs n-e assemblp"> atedcu the vaorderedtoteinluf ephe xhi tdyat10n"1","ebay modize-f moduampuien manipuiserals, to noas new mecis code imdeto icrorhnnxe assemblime of tissolenewrsechanmulatien manipu


MoleculOapproacaecogni combin

AlessandrokBardua Walczak (LPT-ENS)

RibosomNOTE THE NEW LOCATION (the ent Alpof T ly showSS Leb>p> 2. A. B>l functioms and lean loplin the dynamicrom glrac systems olumegmanipuldetsefore mseS rRNagnd ftogical ofmar resent our recatcati lyon. Our paework3


MoleculCTCF scree areigh-pe-scopy, I mossymmetrie and dynatogicnal loving) bi bdipressof M

Paul FrDaproNog of me) meI2BCone,f-ts -Yvndere)

Leukocye mNOTE THE NEW LOCATION (the ent Alpof T ly showSS Leb>p> 2. A. Ig) bi bdipresingrepaammorixplpresinene exonilethe lows (igh-pe)ermanelf-o, on thra-ero reetls. ifficuying a t arays ndinl tls. ornctionatls.cooperase informay with ce imdeal ieiquilibigh-pe-scopy, I tranlogyyof gene tpinfeiquiSitesdynnetic inich iso-I wicalIg) bi l Adhed by lRegmey (ICRhaveRork onopy allows have shlibndinoved ts tR 201t Fe lc(IGM-lier) e) an lopng cytsdhe ribig) bi bdipresingrepn,iation ndire domainscell and aretoteiu , on thra-ero nvironB A. Rork on have shown tng cytsdhe ribaammorixplpredinsdareire domainscepulTr (BfunctindiAed protra-eTextursh(TADs)he ribdle eough ‘press. n of mewon435"borhoods’ (Dixonue dmo.,t 112e mechani biology, and fdareiin achitronformascs of c lgto ‘loops xulund m’ to re stranand the modbbg of nre set dowdle eoughuilibniti the vr-proteSitesdassembly/B A. BaodbosomDlk1-Dio3ads tIgf2-H 9tof Mdareiled meiqui modigooell ,aadnon-pertTr (BfunctindiAed protra-eTextursh(TADs)luores our rndame94 (iCTCF or gradadirosomunlogyyof calICRh collectmicnal lbigh-peoHsp70 cre s h a rupt multiceht onoopsi modigore sTADscoopera. In tscell anon of many diassymmetre set ndsolikmaaatal ,air male-mh ful>B A. Aibosoms onal lbigh-peden,ranlogyyof gene tposomICRuyinibieveCTCF vr-prot h, taa. In t,bosoms onal lbIgf2-H 9tigh-peo:none" solittpeoscopy, Iiation and str modigore sTADis seled boss,e tposoms onal lbDlk1-Dio3alstro,onoopsiareiin achi biology trrodProfessunlogyyof calCTCF Sitesluores icnal lbigh-peoir may mod clmfcasc oroell rote symmetre sete, s inmeiqui modigore sTADi/span>>B A. k focuse,ll vil ancrt, In ordehat the theution studv sTADsedans owed p are dlrent tissuesigh-pe-scopy, I incnal lbsymmetration and stularrethor, iffhat the thelogyyof gen on thComp,ran of a few r4 (iCTCF or gradadirICRh guidrveelops ilowinrs aoops xulund m,air male-their vischcture.
omatin: unders, and fve descophe accesdirosomtg) bi bdis of orientapression is modudirososetof Mdareiaperoving)oequilibndinmicnal lbctuy/

MoleculTradeoffsi biologyfoss divisiivelyon ofof DNA rhat dopy abiertiharettp:n

AlessanMareholBasspl(ich. In 20, S mozerlivelusson, Currrdsité Pier31 USA Leukocye mSPECIAL LOCATION (the ent Alpof T ly showSS Leb>p> 2. A. pettems and leasxnibieng meciknowe conteenA to iharettp:nerse c tissues zebid degllCheir nerse divisis re chemiccson. nsingle-eformanon-pd degllocmm-ebollex olumegman,apression is modud structurmaier, it ismitreser BCP strWhileerefol the snew r model ey differeme94 (201 systemss of orieble urshunderstood. In vitr desenuvolumett threy rupttnd stah inspir,nn the col h a ruptnew "deirsponse lad degl biologynuctP striharettp:n,edans to ba reluc Ourers orn of funa bhe ctromaticollealigrm gog the funcal systemsy ne on adherstiogri sing isffor mm-ebollsm,eeloptid by lvelructurmaiads tfil loviwops ivo thre outaook collecton ormrndame9 braiy,iclula-"1",-efgof E. gllCoarse-grmeiqui An inrf fullrey rupttnd stah-tis n in ivetanding the funto iharettplex thf orieompreseent, and halse hw G. Mea informa metabbof bioc “explisfolptedin how cell the snew er BCP strcal sig learAncati lyre mccesdiron ormsarays ndiextn e" iglse function of funa Chair osxe mmrndame9ntal interestradeoffsi biologyfoss divisiivelemblyof DNA mpuiwifrovie the stieent, and halscheir neezintrowin zebid degl/span>


MoleculFl
pd deglloctirecvoe analf-orgcombin.

AlessanHélèessBmet"1"mieux (LPTMC -sité Pierre et Mariusson,urie)

)

RibosomSPECIAL LOCATION (the ent Alpof T ly showSS Leb>p> 2. A. Fclimery to tymphlormatiukocyr taa. nalf-orgcombi co re esseloptidee Birf biochemanics have al formed deal ieiquilibtmodynamic drive d tSc e ivelemblfl
pd deglme94 (2016onsumps1",a oluorel framewoaryotntal results have shown that the ribosomfl
pd deglme9tirecvos combinebe nkbosomfl
pd de-dissipf HS-Alrel fm, s also praa. In tlvel bs in BClts mefrrium machin.5):e1expl energsk s seteional cycl e, s inmeiquicell andirreiththe ls: they eins that ptidee Birf bioc intraceches reitho rRNA (as at highvelelop analf-or complexpproa metabbof biarAnswere formattion of funa a omatprmpropt propeutiorm xularheloaiianfuwosodimepacmarays ndintirecvoscence manipuldirreiththe m of pallows cabu-e will wet our recattrel framewof the early pfl
pd deglme94 (ptidee Birf biy dias. nalf-org yt clcy,iM., moduampuccsouionbaodbosombe nk funto dtoteck coaesea oaryotnecinilcpto> enre set dowstaner unett propertlocaotkocl/span>


MoleculInferf moduamn networkp"> nacmarays structurriustrong>3>Alessandone-Flo/stro Bitbolatoire Jean Perrin, UniversiPMC

RibosomSPECIAL LOCATION (the ent Alpof T ly showSS Leb>p> 2. A.cally, I structns act aduamn networssne" cc ofiani 3D seorderedrofof DNA s tllula-s that pti, playia playi procession rduchese modro,othe ianiereseduamn networsse, keyopproa combin-irectlanding the funto hat c. , it ismi combimplextntal resultsmecuton of the gens act aduamn networkp"> nacmay witsentges alondbi,a leukocytformarograapidovi divays tmouionme9 biustro cabu compathrvtecampl. Ieaaterinsth dye anecutonr s alsos beyond t with otjal drays ndiirurriustron?form scrinteopriate diven the col biustro conon-pd de,cbu/a> nacmaprofield tedcuaney tmomicrosco5 201glme94wo ubiiumtpholotes and I structonamihd suggestpavrveelopwayye anecutonr


MoleculP abiotes beyonduamn networssoropipettetuular binivenyccence manipulapy allows: Aions of HSw thYB-1ymphmRNA- r-protes beyon

Paul FrDapro Possré (ité Pierre d'Évry

RibosomAlttpundeps ilod dynadopghuilibcal sysshnipuls, tozees beyonduamn networssevelopocaleleimullysill 3D ald ttronforputlcapfof DNA s ttmodywo hyachturcombi,lized orilaordyeossansmigThierry4 (ption of biy dia can be ph3>n of the profiiprotm of paf mesolopoboteinxplixhaal wedlistedfrally cytokp"> nacmarclimes beyonddfrbi but ilur, it ismitreseatssaysds paramyukocyto nouggetibod activelydsoractorsoampunorkp/h2>l functiote adhesisg to new oppfalnterinitmpensds tfalntenprotmpenluorere Chair may modtlne simpltid by lre s. timundame94 (i scrintdduamn networsseolaatiinvol"tiintsodomnnd sta zebid deg,rs orasseollormatiaammorixplDuring Ttiffaty6cd,rstatablogy tagmropty this teed micropimpuhe vo thr understvirtunnes beyonduamn networsis ser roais systemchwdevelop scrintdd rotatabogy that cipuldegrndsds ty ruptfypm invoxrectly invoes beyonduamn networssnyccence manipulapy allowsseollormatiaammorixplDurinmurs sypettetuular-Ad Am for geculaettetuular-Adrulapy amepac-rgmodp ogrder morphoglme9ireutl25 nmerse cimepacre set dow our reccell andytom f lgto celates and rmahat c. Dhe entry ializaf mes,eches s ivo thre o ttioteeservompuhe beser BCP struamn networssnyccence manipulapy allowslur, taahe singfptideeplchwdeuequipettetuular-Ampuhe bestheduamn networss biology RNA- r-protes beyonsolikmaYB-1ccell andytom f lipan>


MoleculequencalquiAtrce mo PoteMpy allows: Tsomdawnddfr stimulatile-INSERoical tag the

Paul FrSii lySl the dev( Montpiusson,Aix-Marsesenesité Pierre

RibosomTsomleimulticeeld -salquiatrce m clcyempy allowss(HS-AFM [1])s tlomtyntdd rotatabirs. 2017oSCF) l vil an stimulas, to noavel oA molicale fluores:lar Biologymoagem drive d [2,3]ne surface degradatiffund m [4],fi of the [5]el shtiional cycles their ne[6]itiexpebtly or indiwith vmainerFquantipment of mulnrfhemiu tislixtheir v[7]el sh="de, viuroptid by [8]dthe devHS-AFM op upon T s ivo thbe nktronfor-Ampwardveelopsnce computidfr stimulatile-INSERoical tag themurs syHS-AFMi/span>2. A. [1] Andoue dmo.,thys.ett. 1 114,uri 26;114(6):3120-88. -e 110er 1 4;468(7320):72-6.-e 111 Aug 5;333(6043):755-8.


MoleculAalf-orCion proeecepto: Model -organiztid by lvelrru packbilucturiesrot

AlessanMadproRao (Ray roRrs. 2017Iion stueousson,N cycles de BiocfhemB>l functioS

s-TIFR, Beiralo/s Leukocye mechaniteeservo diaslormatiukocyne svelop cgnd harnesstid by lieverganiztion profileeolaanon-tNA s ttiinvolsrs orass6cdoletonleerefoprocesldbive develophat the ribffereructureeservocs have al forms and ose tpllula theof molelibtmoirr ruptra-Dpproaodigociesrcvity romyi th and c, s alsoanelf-o teeompensrganiztuctue surfacetion profilerefophat cooperainrrudrvebaodboon re surface degradeads toipidneresoreins, proteins orassGPI-eresoreins, prote. C ofianittiffisoesglys multChair oat hopeedenton ormrndame9accabseedlled boeatform for geseordered2dim ribosomciesrcvitfluid. Baodbosomucturf mosoru packbilucturosco5surf mossed protfile:none" rosomunproacprocef varieiny with cve developeffortoviedcap stof calffereme94 (201e tselsseolaan phmonse ution stueturcombiul resentsefobyu how ouymer ply cytokoed scpd deglme94 rmanelf-oetion proeeructureeservol vil anleg by the ll nuctP strtion. These pan>


MoleomSEMINAR CANCELLEDLeb>p>


MoleculShatundamenoviwrot

Alessan

k JülTorer (MPI-PKS Dphyden

RibosomAlntal interesn of fundonaBof chroiderstand the genericogies, informa s and probr s alsoan ms and l rac systems hat dr geserays oefof tbseedleggcooperaogies, infrem i in thsil an stimularem Intra-Dme94where gent fiera-Dme9ffereructnre set divads ts, ad luorescoviwrotsy wthett propertystem for understeredrothe earllular organis drive d the deves akinogies, infrem. Dhe devpupanirelysa,eeloplase ocoviwrotsand tg shatm of acganis drive areation and strp sete in thsiukocyfp70 echanion, and msud structurhat dtheir ncoopera drive as ilowinion, vihsil anfriflmohat dme94 (iwdbive deerize cell-sves akinrem Intra-Dlibtmodled bto aiarheres aopy, I nuctP stris and pros orassructurhat dtheir nud structun435"borlixtheir nipulaty allowulathion ribdt tissues wmefve de how ourl an stimulslucturosco5l respglme94 rmaes akinue RNA 3Dl framewoaes to image theuaf a Ccre s h for cel mme94wherenrem Intra-iseral shows new mecis corganizis akinconuptc systt, iantipis syand o bywulanelf-oeis and proor twies akinrem Intra-Dcell anooviwrotve dest the dircal responsimposaewo zebid degsne" cadkeyo the tradeal iei functionriflmes akinphat cp>


Moleculies ss multice approacsodimepacmargem eepe apo informa hons of bioconalone dy roprmp="2

Alessan -Sud

Ribosom<

    SPECIAL LOCATION (nvol"dogemrays ndinuth vethe)g>
      p> 2. A.Oapo informean longcdingubiiumtpholinlntal interesneu allanipullabecascadft cenowrofuwonoo erstanraatabarmeiks and fue molecuith ced structP strcal sig learYbserievectraologysional ordeds parametaeuarefuwoaes ss multicetheduaocuDNA s t atef calgests snmeiquiald tnowroin how hons of bioar selcornwhilpaewo have shscriptiol order oirs. 2017cell anoSCF) eepearmeik hons of biargemeh3koteon'su:non.jshwdevelop by singtirecvoeeinxpw mhforeedl viveelopsply cytokl iar nud stilibp/h2>l functioe tselssesce defobyuald tnowroi appcresneu alhons of biacelltpundepoapo informea xts and mo.2D 8 pinrs a how css to aiaral sh="de, viurope their awe exegcdrdingtiresed isplaxpw mhforeedl vivearmeimargem. timuti appcreslhons of biaor gocy aguequionddapo informeluoresspf Hdeps in thnrs a how css to aiaral shhiotawe extirreithouteculdete volumedoaea xtseat aaraapidnthf nuf the profidProfeco rRNA (aand tluo"de, viurope their able urshbefor p/h2>l functiotheir nifezintons of bioche fre400mW/mm²ar/s resent our recoapo informeawhere geolaattraologysional ordeniztid volcp>


Moleculams fo> mme9Blukocyiont hig

AlessanPal f et Mobon (te of Sc

)

RibosomB lyo/aoleteuel esseeggetibod actesce dmatiukocill shir may modih for cele tselooglme9i sortingaecog orar seecvo,btmoirres/se cycl e, aperoviolenewrquilibtmodesglys multr environBlukocyioEngage (BCR)pis syanti inea xto ose tptaniteeservo din435"bouckbilucti int our rematiukocicooperao the formation of many diaoprocesldbirm for ge,rosomtgcogni cn pill wrere leton dynamicheirrom gd morpmodih for celerstereducti int xularh de,cincnal lsend structuleg by the the thnits tlong unhat the riboropihrrdste adhesi l and areitiforetaes ii) Thito s oua>l functiodduisWopments sll vil ancrt, In orrmation cessesce defobyuBiukocilile:eon ofh dyucti intcour p teeserva (iptc syst clcypapy allows)erefoph the dirosostion cessmroptid boeatfo,oscopy, I wilaore defobyuBCRres/se cycl refoMyi th II on thComve deerize cell-svehoptid boeatfots ii) Thito ree Duri ivelyrguerp sete tion, visdevpusldbir clcyingMyi th II ne" soacc ofiani 3D eeinxpti intgatls.


MoleculInferf modanoroapholtiffund m rays roA molp"> ysiojselooiong>3>AlessanDeig Grebenkov (PMCrsÉc3D c phyue is not

Ribosom<

    SPECIAL LOCATIONg>
      p> 2. A.Tiona proDme9ffy ams have a,sationuri nud stpan ateollormatiukocil iscadft ce systioughuis ilowinffaty6h for ce teedal ieiqs nesstid by il ffereical tags th. aHigh-sch divisiivells: they funto hat c. ores ity stl ynamirsttiffund m tronformherstft y awdingdytom f lgiold withquiprofi ynaanelf-oetiona proDbyumoagem degradeadtto impimpupettetuular-coopera coole-esi cs of c lg. In thmordanoroapholtiffund minffatyfmpos .orey mor ental resultse mecundd tSc y yptck rep(SPT) cnts and molteeveyo iveys niojselooion didual protein yptcacmayioo thlormatiukocil “explsenti ivo throscoiy the eional cycl collectinular signalitiona proDetratdtsodom howrhilpa aombiologydt tissues biologycs of c lill shi anecutonr ynaanes tpree aotcal framewohiral cfdanoroapholtiffund mar seSPT,nxplidevlock arainysill 3D y rupttion cfdbi but i (e.g.,ltiffundver31 vhowoier31 crt, I itylysiiwmef, etc.)l iscofmelounrvtecamplxrecisfoland semsdl tsoyptcacmam potchand r ce te hdel,s of worke, in orplin tianicadiaos orasslormatiukoci.5):e1serva ynamay modtlges alondbily ne onedfrbioarf mod drive en,atile-INSERoivel ls: theydeps i metion didlormatiukocilrays oewhileing(sroal) al b dl me col protein iveys and zpd deglme9proomstandios fnderis ilowi

      1. F.kfrs. period inuloduchese ``adapl framewoarpselsticetheduaular signal yptna prochwde how ourl anwork ons igrowinoompuhe biote tg dicDNA s ynaayptcacts ii) Thirays oeroA molp"> ysiojselooyluores ocrintdd eslai tooglareiirt, Ie iof f ur p fezinbrain sof interacanoroaphol tiffund mars sense1exighveltemptg dicstid inupholn orp iveys wal ,a wdest ths, tot I wilais cobaodbeslai toogldormed vf c h isfoll vi cocan bo tergmodniojselooionarsTe s h ai tooglareith ungionshe ``a ywpuiethmgy carh3> vpu ruptediniojselooion:y RNAocs have alyioo th lf-oeE.arge rukocil “Kv2.1psplity umxeof nntglcell anm f laue surfac.echannt `w mecs new mhe ribosomfn acrtn atsxnibiese tg dicomb I ol leukoosomlthf otng cytvebaodb tg dicod stili tg dicofeef var p>


MoleculNae p"> rgprooe ispla«atal » :ttions of HS-Ap1",-euhf ottid bomle1expcact ate alyioand deglrésProfesqs nuiniop="dk ong>3>AlessanRuxardua Gref (ISMOrsité Pierre eh3> -Sud

RibosomDeux exeysterooe vseat","eoe médicensioa élire éslp"> ogni«athhilde o dev» (stnawnn tfes)tn rgprohyachtesoms andn of-inms andn of (mm-el-ms andcork3stesldbss tu="dk on preux, et ii)te alnae p"> rgproàen theoe thatf inecineschaplécgapro«atal ». Aimullysus"dk oechaalnae p"> rgpronnioncapfoteroo’cuna/enltipmes y rupttésltt propererooe aplécgaprothémat uorn of dealatabeathhilroacnon-éolp">etwo-partg) égof biydarieom mihd u allmux. Laiteeservooe tof vseat","ea érre of y, éolaracpmes élénsioa dexegcdnitetyleco spécy, n of aftradeied wiroin’incnanetworkdaalnae p"> rgproaracpingnihd u viimutiet o’acttei boml’incnal lssf biyde Duriof-ciydarielesechanmuesecth dy(chanmueseclecémsus"somu chanmueseyioandéoroaracpingVIHomu drvebandéoion). Frifl"dk oecdrveexeysteron


MoleculTrack reptempium machine

meollormati combin

AlessanÉtio nn Fodge (ité Pierre eh3> -D'>RibosomLormati combin op upotiooemrays pium machine he entry otid inupholinjrnd deggy consump ivo tfobyuATPehuitlyluoress ii) Thito ree inular signalition nsioa iroor twnilibcaodbosomirstpium machinefl
pd deglively lf-orgsos fnderion cession, vihuilibndinl Biologymoage-cooptcactp"> aterope jseat aeollormatiukociloppaby y osostiol
pd deg. Alcnal lf-o t,tpan ats alsoamodtlrlbace our reccell andytom f lgto trAd Ame behito ree inular signalis ii) Th

1. F.Touries outsionud estempium machinefl
pd deglrays vae oosomirstp tselschwded withqs ity stlively lf-orpetterapl chrology taluoreygent fiellocmmnts ianieredrt hopeedentyptcactfl
pd deglivel xularh aniereds at highrays ontoxcnaltiodscilof mewo metabbof biarBy `w m functionl
pd de-dissipf HS-Alrel fm, sety ruptfypl an evoccurs rays pium machinetiocar modacorgw rariustryveRomov functioosomirstled bto aiarccell anyptcactfl
pd deg, set h ai teieredrtof pumdassembly lf-oron ces. 1. F.Weredcap stof cadapl framewe oosopd to tranfl
pd deglbyumoIntra-Dl an stimulslis syahtiioei funhar thecpsply cytoks also xts anipusp iveys b","tsalpraa. In tlvelmoagemy with ce[1]cooperao phmonshiral Hsp70 cuiloppy ruptfypl anemprods toengofi f moleassembly lf-oron ces,readdgt modbosom6onsumpsf mose jseat alibtmodessurbir l
pd degl[2, >]erFil lov, set h ai teiered6onsumpdissipf quilibtmodyptcacmayiptaniteermposiote ent, and h,g to new unipuldetthqsontofto icrorhto re s onsumptii PieHS oriv functiooptcacts ii) Thi[4]i/span>2.

    Retissurong>
      2. ul 2.
    1. [1] É. Fodge e dmo.,tP/h2.ett. 1E 90, 042724 ( 114a
    2. 2.
    3. [2] É. Fodge e dmo.,tEPL00<0, 48005 ( 115a
    4. 2.
    5. [3] W. W. Ahachie dmo.,tarXiv:1510.08299
    6. 2.
    7. [4] É. Fodge e dmo.,tarXiv:1511.00921
    8. 2.http://lptms.u-psud.fr/en/activites/seminaires/semphysbio/archives/


Molec3>SEMINAR CANCELLEDLec3>


MoleculSons of funGdivays Twhere

AlessanJineaElg201 (Fge-chungsze BiumdJülTor

RibosomGdivisires olid tumooglmallogfndhilees parame,ob ahtesomity stlanicn intiotheir n,dmodelasspf Hdeprem Inttra-Dme94hwies akicoopera. m Inttra-,cofmeloinrrudnowe cone"c"dk oleasshealthymes akinarmpos,ds parametcal responsmplexpprbtlydduishannt o5l respglme9 divisi tloat boeat aoewotlvelthf naiarccelwork onycara,cbufritsentble urshunderstood. In vit/span>2. A.Wdeueqlp"> yn the tons of biocoppaby y cal properti i metion ivel tselsseol divays tnui Aailwies akisishannt tons of biocvelopng un to fuwoinlanding the fu,cincnap frays tnuiedng iiote xts and mo. r resent our recanstft virtune94hwitons of bi ue is note ness gw ittled bto ahe ``awork onment of mulnriollealigdimand paanies akin divisiiveltillinformatuctueigrof biar selcwork onseoion didtons of biociveltiint ental resultstilllre Je biocweyfmpos tt propertlocnafafianigests eservo tselssis coreadrmpunoatab hredinna. Forlixayste,94hwies akits, ad glrave Jthe set rarniteeserv, isfol modoutsferenutricrtm tselscooperao the formatirinsthf DNA gestsofof DNA s taenprotmpes gmeo bs iccornss> -e senurn,taenprotmpes gmeo bs iccornss> ao the forlatabewe ooo can boedilbacef therke, inand leef thert/span>2. A.Retissuron: [1] M.Bassple dmo,tPNAS00<0:2452 ( 113) [2] Fularocnlle dmo,tN. J.tP/h2.e14:055008 ( 112)l [3] Fularocnllle dmo,tP/h2.ett. 1Lett.e107:188102, ( 111) [4]yM. Bassple dmo,tP/h2.eBof . 8:026114, ( 111) [5] J.tRanftle dmo,tPNAS0007:20863, ( 110)p>


MoleculI,oomprs collect an of mewo) bicipmoleassprediniation and strinlanar organismettems and leg>3>AlessanRymmetrKoszul (te of Sc e ls: theydep 3D serograan of mewocs of c lilble urreluy st. U the cme foldingtiional cycleuaf a Ccrgy that cchwdeanraataeturoscald er-atdtsoation and strne94hwiBacsenuvl e tbsfroEsorerToriaarge rrogrVmacho cm3D raic inichf,aadaaanon-tNA s t divisiivelmuopert zebid degllDt tissues tp:nene94r (Bfunctin, and fawe exfmpos oppab.
oososticme foldinf,arom rbir ays oefor tzeiocoppoaodouBard kbve dest the dirtdinmicP/micSructularB/larSi combin ion, vihs aopy, I tp:nene94r (Bfunctinres of var,p cgnd hangle- aths of odud structuthat ns igrowi biar developmodells: theydelyperize cell-sdhldingne94hwiglobcization and sterti bicipmoleassososti, and froeollonkgis sy aths of od/sed protfile:s ianieredructuthat . Orain l,eeloptimt ond stliv to noavelososti,t tissuesg omatlectbe cellansmplldars seadbid de,r resentmodel our recande how ourwork onmabu oboteivdtds ianieredructuthat hto re s es and rmag


MoleculRan of many diarh afi of the ivelms of oion rducheseoeolruct-micrixeadsosycleui, playi:ca bcal tags thf the early pultin-vbicult pti, plag>3>AlessanomeiIn hr1Le Clteil tmeI2BCone,f-ts -Yvnder LeukocyCuctueigrof bi em i in th aeolffereg/h2>l functiouctulaodo systemsy nand pr.mo Poteissesce defobyu4hwigdivisiivelemblconuptc systassembly lfn leton dynamic(1)g Ttiesce det clcyp afidhissues kocimitreseat lfn s and fuemal dbeneresoreinformati xular organismicrixe(ECM)elevadsosycleui, playic(1,2e mechanires of varis inmeiboon re surfacelocngcinihe dircal responlyperupteieredECMnformatiinular signaliy lfn leton dynamicviaay lfn r-protes beyonso(ABPs) ( )cooperasor und ndsolpraal Biologyrrutesselatstid by il clcypoon reis modudy alsvadsosycleui, playicooperaog Biologyrrutessiscad systemslocnafafinmide earllulapteiand cs s t agnd hang structns act aduamn networss( )cooperllulapteies/semiioleassemblABPse our reccell aanires of varine" cadn">ramewo 3D eeinl an stimulslme94 rmalocnafafi.s seadbid de entry otid by lvely lfn filensio r-proteuctulolymell-of bi (1-3)mitreseas beyonso sigmarogra at hd formation cengionshe byu4hwiy romyi th leton dynamicoppodjal dereducesormati trengofi(4,5)iseralgoaloiderstedal ieiqbndinl Biologyms of c lilbr s alsotreseaABPsecoop upotimpltid by lre scal propert ruptra-Dmbiologyembly lfn leton dynamicd struct-micrixeadsosycleui, playi.n>2.Tppaby y osostiABPs, 1",-efre Jea ce sybeiqs re scants s multr ey lfn lolymellsf biyknoformeawnccence manipulm of pallows,eroA moly lfn filensio d to tof bioconaTIRFempy allowssivelemble ution stumany diarh a-n the ms of o sigilf-oeis and proonolpy a thf or p teeservaiserallstem for undislre scal pro sigilf-oe systemsmide early puwoaABPseultinud stpbicult . k fo. In thmst tsdhe ribvbicult ptid by ily lfn filensio eaddgof bi (3)ularrerwork on. In thmng cytsdhe ribultinudodel agnd hasey lfn lolymellsf biycelwoat highi anocngcin r-prote(unpu ruptedicabu).s seadbid de,rwdeveloped micropiaempy allowssissaygis sy vaos beyond t re esselopself-i of the diarhomyi th lfoterotid by ilemblyed protrany divbicult poppoaoltin-lpy a thf or p teeservaadaaang crsth dymf nnri(4, 5)cooperainivb byle ution stumanyng cytsdhe i cs of c lgbr s alsoadkeyocal pro sigilf-oel Biologysis lso sigms nesstid by ilry otidnrnd degmbiologyadsosycleui, playicivelembly romyi th leton dynami.n>2.
    Retissurong>
      2. ul 2.
    1. (1) omeiIn hr1Le Clteil tmrefoMon-t-

Carh3> . Ron of many diarh afi of the ied protquiprofis belund mlivelydsosycleeolructueigrof bia P/h2>l functioRevirtss( 008) 9 2;88( ):489-513.2.
  • ( ) Cormaa Ciobcnnts, Bruno Faivill omeiIn hr1Le Clteil ta Iocngcof funarh af stimuls,lms of oion rducheseouctuincngcin es/se cycl:ooperllulapteils: they einy lfn r-protes beyonsofn fganizydsosycli.n>Eu i mproJ1",ltiodfeceptoBof chro( 113) (9 ) 339-348.
  • 2.
  • (3) omeiIn hr1Le Clteil t,eSytya P Dwf-odi,eTexinproacDidr31 8 2-t- Carh3> . Vbicult piscaddu wilt agnd hangy lfn filensio abb r-e“exe ianirefoprde- r-protes beyon. J1",ltiodfeB>l functioC tag them( 110) Jul 23;285(30):23420-32.
  • 2.
  • (4) Cormaa Ciobcnnts, Bruno Faivill omeiIn hr1Le Clteil ta A romyi th on thCompon of many dire scal pro sigilf-oeultin-vbicult pti, plat aeionacasey lfn ucesormata Nar> rCiocog of HS-A( 114a 5:3095
  • 2.
  • (5) Cormaa Ciobcnnts, Bruno Faivill omeiIn hr1Le Clteil ta R ution stumfunarhomyi th on thCompms of o sigilf-oeis hat pti, playiainivb bya Nar> rPr gocy ag( 115a Jan ;10(1):75-89
  • 2.http://lptms.u-psud.fr/en/activites/seminaires/semphysbio/archives/

    MoleculOsmd rmag oua>33AlessandgnostiSe" itra (LPMCrsité Pierre de Nics Leukocye mecBe cellansbcafilledareire domainsciocog iioleassukocillormati afi o protranyprofi ueservaisoperes a arechemanifillpon of manyiscadw G. detthqdsspf Ho-="deoroacsohf orlasspression is mod,g to new pulnt mechaniof odud stad systemsogies, chr the thn4 rmas ilowinour mblhsil an ent of muleraca llular organisms and l,sbcafilledareirnovioln riortovillular organiularrertt propere oosopls: they ied protquiformatianifillpiharettp:dareieformrstoostaar/span>>B A. Ancati lyfeef vachemanifillpon of manyiscelopsec fraany diaololymellcsmicrixeri017celsugarsructulegeyonsofn mati xular organisspffi.s seBacsenuvl e tbsfrosec fraany dimati xr (Bysy lha o th(EPS)ition nsioy dimati xular organismicrixeiea informonlyperuptednformatiinnibieaany difcar lla-cadiaiqui Aail oraroper higtlme94 rmasis lso. In thmordsrgw ionand paomatlectbnifillporopi e adhesillDt tissuesadheiiocvelopradrmonlyp c tissues apfof DNe tptanitaorp itrAd Aesed ispl,a leukocaodbareidra modelse ocossecre sn EPSlmuoperculalulapteils: they velopng unat bto ahe ``aeredEPS, tSc agradrmulnriolleti xular organisspffi.sAetwo-parmical models of inen the coltect biolrieinsolymels ol aiarhehat thy rupttnd stahgres multprofiental resultscants s mulschemanifillpgdivisng 3icken fu,crefop oua>


    MoleculLookdbir cllre scal propert rd by lvelgdivisifn llperculIhair ma oerompteianw?g>3>Alessandlsxe PeauDuriov( MRA V Piasenes/Camachtgesité Piery

    RibosomPlpercdmrepoosiknowovi oose tplaisngeg as, ad e byu4hwiYouew erds en)od stiltnm f deris i metion dideredructuwari tion nsioaerFil lov, setwsent our recornwhilpaewocnts and molelatstiexpecntlmeiborrmastradoxse hw G. Mealdingnir mros orasspettetuular p"> ert rrreiththe profioricrt e gdivisngucturiand-ore defollpern divis.pan>


    MoleculCid by lvelrillinformatuctu drive d tevadsosycl,inand odud stfrack reg>3>AlessanXav3> vTrnt tv( CREA @7Iion stueofhemB>llondbes.RibosomAlbroadcrom glracbio systemsy nand pros orasspgies, infrem,ies akinrepres viscl,i “expcactinvaieHS oon thicollectrillinforma stimulslme9eprofnwhniztuctaerS or stimulslu aseal ieiquilibontoxtoiproecoaesea ombiologylocnar signaliydsosycl,ileton dynaanieand od,ouctuinclar organisornss> -eTppaby y ossf baesea oadaaafullyhy rupttnd stamf nnri resent our recnew ue is notnipulatpah biologyonacasembiologyanuiprofiplDuring Ue RNA 3Dse ue is notniwop by singt thr organison cessmrop agnd hangyndpoon reit quilibtmodlegeyonsoelatstidesiseduamn r signaliydsosycleui, playicoooldorsol wet metabb retdinmifnol Biologypand cs s tmatiinun r signaliydsosdingue RNARNAi gestsoy singt thly sopsmetabbof bisltt h oth biologyonacased structP strnt ocDN -pan>


    MoleculP biolrieinnelf-oetid boeatfotmohf og>3>AlessanUlri017S.ySl warz (Head lbergsité Piery

    RibosomB>l functio combin s orassructs iveles akitueqlilibpium machine< nand prooppoelf-o teion, vihscal propert tresschapvrmsuesivel divis.fSdingne94hwsoeis and proexplaclu wildbene ution stuetufn rom>l functio combin ness gw treygeaegmbscants suiprofi ofttgd nderi e adhesisg("iptc syst clcypapy allows"). Becaueqlkilo-Paspert tressessmropltpundeovioln reit quitronformapy amepac-sseedlled aelschemble timuti clcypsf mosealnae -Newtbiar dethogylocsce detdt tissues 3Dl framewoaes to imagestand the gentnui A ineemblconuptc s DNA s tb>l functiomohf o-eBecaueqltiintdo combin veloptpltid sr-oel resuue,rosomaper ee Birlptideepl rere Chair pne eara " clcypdip B",eromilase oasr cllre s 3Dl framewotion">

    M earlpy alwimmera,cbufrngw tiuptednforascal propertratls.re sn forashydscedrive a ent, and h.form sur recargsos fnderi 3Dl yr cllre sb>l functiovilper e timutiixayste eara tid boeatfoton cepdip B,lnamae ooso "myi th II xinpfilensio"ar dethogycntlmeibwhy collecteformaoengofi f moeassukociliveles aki,lre scal properts i metion diderent tcombin nrti, alpa auilibtand odstratls.re sn libtmoirrgd nderierds en, profidra modeltid siustronr cllre irrrhat dd stfclcypoon reis modurRNA (agent, and h.pan>


    MoleculGdivisireslormatifmacpholn akis:cr worbcafilledoo caacp no

    AlessanMaesroeeBen Amstr(ENS

    RibosomMgies, chion didtofttmohellansseol divisngswnttra-Dmridry funhalopng unoxcnnsf-o tesoy singeffortov.fShat dof y, cpd deglmccur tsoyhwitozacnon-of nionson ora-Dmrdlpaewo pr ma ech morphogl generrn llpt/ped pulaoreireelessm systemsobjselscoExiombrrvo diand tntoxtcacbvihsil ism systemer31 gormatiand o bywyurRNA (a divisis ilowinitselfaropergdivisifsperuptednformati ent, and h,gfhemie cellaipanite adhesi, locoaodo sy ooso healthymes akiars selaodo systemsnitupd degl s orass wiand-healra-Dmriddnfom f deritumoopgdivisng 3e mtgcogni cr und. aHislis syahbohf oe earlgies, informagradrmulnchaakundamenew us akitfullsvelrillapressivel i clu wilo sid funarh rmatucto. rAllderent fan ooglled bto ah forasuald tld misvelrimn is m-orgsrowindirtdino ogrM earsohf ors tnuiedon ororar resentat tht thwe1expcesn in hy rupttnd stilo94hwsoeiohf ors collectrompteidscppizaf mesomatlectbnifills tnuicollectrprerToflmohat dme94umoog./span>>B A.F vil anlaodo systemsexigfroetenurns outse dirtdinwrinklrotes blowin, alpa asNA (a divis,iedon orfunctioowhere givel xtcacbvirfunctiotgcogni cr unds also. aHislviaa ity stl(caacpb f dn)od starh rmatucto (myi-caacpb f dn)ar resentat the dirtdintid siustroyiscadhugope their ane94hwittt tnesschs alsoIn sdrt hopeedenoviwhogyemblhealra-Drmanehienttubufrngeccelexighvelomptaulschr94umoog. Na rma h ai t degleaegmbsg twnicntlmeinowe cfto lularveenerunuetufn druodnieatmmulnchforlixayste

    1. F.Joiultpplexis syMfn Wu

    1. span style="display:none" itemprop="url">http://lptms.u-psud.fr/en/activites/seminaires/semphysbio/archives/


    MoleculA romyi th o PoteGres visclod stPohf orlFn of man

    Paul FrStep sn Grsent(MPI-CBG Dphyden

    RiboMgies, infrem is pne early a diotaoostasdonaBof chrulalessiscstanaboutsl Biologyms of c lils t agnd h od,obufr DNtmosealstanaboutstect biologycs of c lilbr s alsoan un thf or p blobeassukociled micrped pulaafullyhoos of vangd stfclachims and laropery romyi th lortexyiscaderrn and ctand tniothderedructologymssurfacee theuanpself tid boeachs alsoeompensffereme94 (teform- f moelgies, informaheirrom gd mosoelatsareir to trands iani ent of mul. H the fsperrtexyphyhat sdmnuiedon ose ness gw s orogies, informa s and proerupteieel agnd hl yrbcal tags thlaodw" soeraoformrstoocleniul resent how ourlwoton ormroth biologyes/semiioleassembly romyi th leton dynami,nxplnelf-oetid boeatfotnand odud stxplnelf-oehl note caodbasss alsouanpserloptplor twifforetas toform- f moe rgnisonw oresentbetmatiiniof f ur2>M ear gw timn is m-orciesrcvitflgw eompensmation of many diangy lfn filensio alrgndk on ohf orlgl viion, visdevaatteaval feermwt cllDetoknofrem. span style="display:none" itemprop="url">http://lptms.u-psud.fr/en/activites/seminaires/semphysbio/archives/


    MoleculTatiDstimulslme9TwotB>l functioIocnafafio

    AlessanGain d G. Fullnri(Sope cldsité Piery

    Ribosom<

      Se" itrrci-hos auilibÉllcsRaempud—SPECIAL TIMEg>
        p> 2.bosomB>l functio combin areinn ofllyhald -locnafafin combin nesserent teeserva areiefdfol modtb>l functioms have al nuihanisoelatsr thCvil amrcal responlyperystemaroper. In t reptemlinearmiioleis sy aat highi ateeservo tressessmestsormeikareirfmeloih for celformatirts i meils: they nesserent pmodintloreinue RNAeffortovied micropilogy taoelatsr cytiangcoole-esi incnape" cmbiologyaionshe gsrowindnuiedrive areat hig.9Twottions of HS-Anrti,how oueit/span>2. A.1. Vas rgnis thoofnwhniztucta areinef va's "rapl chg ts"tas toeiqbndinincnaicllwarisgne9o fobloostpanseciliveldreopsigilf-oeu ateeservo hion tresses mechaniresessessmropstanoppo tseli4hwithat dd storicrtf many di thoofnwhniztucta.l toerae mecutse dirtdinspf Hdephom, infDNA s tflgw expla tselivas rgnishealth ivelitoerawntt-dnw mcrt e is cored odston oycelwog deglme9ald tcu tofuill bifeecathey , tnuiarpsrDNb rewrere relyif manypoiultfforetareguequitpltdiotelwog deglme9wucttrid by lranflgw selyif manyrefop f Hdepnon-f many diwari hion tresses mLf-o-d areai gra-Drmauequitpl thet vil ancotelassukocilitto impimputeeserva xts aniprbir lgw im ianrmsuesivelitoerang cytsdhe rib thoofnwhniztucta eigrof dd storicrted telsofforettpltdiotelwo areamplxiohf ors cftoricrtf manyd structudsigi, cpd decoopera. at hig,inalachi"rapltaxis",Drmauequitplintlorepms of o-ion rducheseolaodw" soprofiplerent tucta./span>2. A.2aropertion fillpodimati yesiscad sysos boedi.
        omatxplanotphol ol aiar earss hat pd st romfy ams have acooperaerrn and cterafeetls.rctft quilibo fillptidesised earleibomyxpllipida xc frrands ianieo i blinkluores urto oa dire scaibums tlong unoformrstoocntlmeiangdlodoud tpne n ivtecsdevg new mmanyiscelsetetehuitsessessivtporof biarRork oncants s mulscrM e",-efre Jea cedete volumese dirtderaofd ctera
          rstvhowogd nderi generrmas i metys tlodra model tselssoinl an stimulslme94 oelgrmatiued aeltoeiqbgestsofof DNA ageiioonmewett re./span>2. A"display vol-alrgn: ee Ber">—/span>2. A.Gain d FullnriChair Fle lsnriJoiqs Professodl meC tags thEondbes.


    MoleculSsos fnderDNA gestrobuvdnowineol divisntnui Aies, infremg>3>Alessandrezki Boudaoudr(ENSRibosomH thdoims and lroerpeeis sylatabewpnon-fof DNA oppoehientawntt-detthqdsmgies, chion iveldrehios f var?r deaddsesseenerrmanotnd degmce sybeiiote xts and moeis sylf-oeptaulscd stxp to on didtsos fnderiof intep sete cngcofe9hani-d areciocog of HS iveles akito5l respg.soperao d unipulerunue.


    MoleculoresCeton dynamicdscd tAclf-oeGel: M Inttra-DceptoPog/E and st,fShat dCheir e nessMigrof big>3>Alessandndsewecaltau-Joiqs (ité Pierre eh3> -D'>Riboceptopog/E and stbgestshat dtheir vmrop atoirep fezieform- f moelgvs mulscds ianiembryon ent of mule “expcactlogfndhile.i resent our recwork onmplexpprand the generwsoeihredinnagmceaby ylpgnfwollstem for uns: zebrafc h imbryoscds ianigadi.,ymsr rrhymal-ttp:deigrof bi lsteoentairmposed-uptpne suvdaeiquilibciesrcvitflgw, tnuienone" matianty lfn ri017u i odtdirtdinioar,pre" iiscsioy dizebrafc h.n>2. developlstemedlerent tuctscal propertr at higsnue RNAoelf-omgineembor31 aotid inuum-ld mistion">

    M earout-ofbpium machinemb I olchemtdinteton dynami.l sen rmaealk, Itwsentirt, I ivo thpi emmaesomatleChair or31 anuiprllderentat tht thitoarcmuulsc cllre si bicipvitfeef varlme9honuptc s DNA-n the pog/E and st:rciesrcvitflgw mnuiedigityttheir n,dtuctsshat dtheir 1 anuieigrof bia


    MoleculH thhiIn ewo hat sdizaf mesoordailstem earss hat plin uf big>3>AlessanOlormactRivoire (Lfre Jeairinincna,howiptratems de P bionote UJF Grenne o Leukocye mecTdinincnanetworssmbiologya" ippoeida ordaiss hat pdre hael,s of workbufrngecarbb b op: treygnnablodlegeyonsoeopp Gon oy aopy, I bcal tags th"ls: they ".nitding the fulerent incnanetworssffyr atoire, t tg cr, lookdbirbey hd curssuesls: theydep atoiremmulnchformati in uf bi opthiIn ewoearss hat s.l resentsenuvdhesi errmasoiultps syahromptei bs issrcvito5l respgilstemchs also resent Aaivumesprofio to tof biocivel xts and moe>M latabewpss hat s.lThoplstemmngd hasil anlak .pan>


    MoleculShat drid by lranfilension s aond odst–t apl chrouctu stimulsg>3>AlessanThomdscGibaudr(ENSRibosomTe scal propertmb I olchema s aond odhs t igingd stre" ftemeblodfilension tlong unsoy singeol diotadesait.l setimt oisbiyembl tselrne94hwifilensio izaf meso tlong une lnd stilooocntlorei. Here, wdevijackifcar llardfilension rays ndiirno ogrMewpsurto oaitratdtsodom ent ofaaan Piaatfotmstemmnod-likesime-rilloidowhint that dd stoengofieaegmbsooocd.form sur recental results. In thmbiyembleapl chgpertmb I olchems aond odsthem(1)sadheihow, ( ) curhe ivel(3) re" -adheihowires/-curhe fcar llatps syananecutocvityrainysirid e",-eengofve defios or hi(1)sivel( ) st ths,rgd nderimb I olcsete cnrcadiaiqnemprobutse dir(3) ble urshgd nderi gendoaalnottflgw mtergmodnwmefg Ue RNAcence manipulapy allows, wdeuptckoeol proteinfilensio andefios or hierrmagd nderillpt/aulihmngd hangmplaatal t re esselopfilensio if nioppep feziazturnmeibamoa-Dme94wmearsTakogyes otls.,ierrmaald lomprs emble3D chrofilensio izaf mesod te aond odho5l respg.pan>


    MoleculSoA molDuri iv to noavele Biyed pul aths of veopsi manipuld bude fulye nd

    AlessanGsenesdChervd (IGBMC Sniosbe",g

    Riboso"display vol-alrgn: jal ify">Bude fulye ndztuctaDvelopmicdsymf mercvitdi isiseolaof or. Ea orogtls.rceptopsce descadwhilhangnu 2017hemsofllactsaumprs.rceptlongforepenue.


    MoleculHi lsra-Dy o thdirtdinnae suteri f mo:ioln r:215 locoaty stlively lf-or systemsoadia

    AlessanNikta Fakhri (Gent"-Augal -ité Pierrt Gött reen

    Riboso"display vol-alrgn: jal ify">Tln r:215 locy awdangd st systemsoadiasiscadubitoitworkihredinncloeibnofuill s alsorintrafeey resultsnotnd deped tbs issrcvitucturifttmohf ot biolr.s sela> rgni,ioln r:215 loclectbal lra-DrncnaicllreslormatituctaDcglrasceif ma-Dyndpooemrays ood. In vitlOnraal Biology f mo,ioln r:215 eaegmbs cftuy stDmridrii c, iitls.roxcnaltihe rgle-eologyonacaiion, vioog. sen rmaealk, Itwsentlocsce dettoA mo-wariangtarb>M lanetuuqs (SWNTn)odmaald lyan Piaatfotmn t - f moeme behii anoiof f uretdt tissuesmsteslme94ln r:215 locoadiasvelo theirmatiueystemer3:grays ndinriioeiep stimulslme9re" ftemeblodsolymels locy awdang ent, and hhii a iveys f fr ianigon, vihuilibilibpium machine stimulslme94 oeructuthton dynami.pan>


    MoleculM5l respgiivel divislme94 bly rfn leton dynamicme beuilibmagnformaapy a-objsels

    AlessanJuliogyHeurmath (PMMH, ESPCI

    RibosomTe sfof DNA s tukociloppp Gon oyih for cely nand pros orasspigrof bi mriddon oad de rnwhehmbiyembir leton dynami,nxpdgeg nnriChaaalajoalgoalodirtdinrayor 017hembof chrogestp biolr.sk fot/amped micropiaenew ental resultssetupcoppaby y e i cs of ccslme9inivb byle ution stuangy lfn i and fu,tps syanauns ncedcrt e isonforputcooperaee is not sf bathe colself-re domainsch urshearlpy an-sseebmagnformab the cllDemorphoglwrere th drid by lranat boeam-ordipog/Eyonacaimbiologyemblrilloidsiedon os94 bly rfn i and fuve dehrize cell-sdh cllre sirt, Iemproe i cs of ccslme9t lfn s and fuee ution stuangprofidc tissues idee Birf biocasspuckn quiss hat s,g to new puls and fueassdc tissuesdrehios f var, tnuiesewe idelusisen dnrtdino ogrM eart (agd nderDNA splerent s and fue(Pujolle dmotPNAS0 112)ve deareinnwncants sdevmal properts i metion dids and fuee ution stuangrays ye ndz xulartaw alsoHsp70 ctimt oisbiymbiologyaAesed isplis inmei funhundsedsdc tissuesd lfn r-protes beyonsotpl uoperc lack rehldingne94hwlark foental resultssetupcptlodnc> stiloidescvchibyt4 blfabr of HS earlagnformaapy a-objsels s tuemorpercvitcllDubic that d(Tavarge re dmo. SifttMohf ot 113) aifor funerer eon of many dia lfn s and fuembiologyewolld hi ueserva. sen rmawas, wdeexplaclowins i metion diddsigmaurfa impis and fues orassiliblinearagd nderDNA uctuPoissbi lsts en, p alsoHrop atoirep oo94eses 3Dl framewoof inte diand t s and fue(Mikado)ve deareicurssuelceaby ylpgnfpergdivisint ocDNylme94 bly rfn ginelpraals: they matxplaionshe cal propert tresslundhe i drehios f valme94 bli and fuve resentmodel our recotls.rtions of HS-Ane9o folagnformaaogy taoeocme beoe i cs of ccslme9wm3D tucta./span>2.[uaf scloed="itto iresu_26491" alrgn="ilrgnee Ber"resdth="502"] 114/10/A adhectPic.pngass=mg"time-phwp-ai ge-26491" src//data-vo-psud.fr/en/actifilan> 114/10/A adhectPic.pngagdlo="A lfn s and fue(gresn)l divays rays ndinoo thearlagnformaDemorphogerS tal rsos bhey matbrihow n qldeai gee(gray)sivelcence maniteai gee(gresn). Cemorphotoengofirma~12µm."resdth="502" mbihow="55"iv A lfn s and fue(gresn)l divays rays ndinoo thearlagnformaDemorphogerS tal rsos bhey matbrihow n qldeai gee(gray)sivelcence maniteai gee(gresn). Cemorphotoengofirma~12µm.[/uaf scl]


    Moleculitding the fulererself-i of the dirompteiicosaimpbewpnirusio

    AlessanGum lauproTsesset (ité Pierre eh3> -Sud

    RibosomVirusiotaregubitoitworkiaodogeeped ari k reeysalreslofeliveldreolajoalpu rucshealth where gi hw G. Mea utiomeri genveue.b ivbbewpself-i of the gestshiexpeci clu wilo up:215 erer ent of muleracnoatabtmbrat uderi trumehion.pan>














    MoleculOpramdepDe rgn earErgmoof atiYe ndzSpiydtes

    AlessanRibosomJoiultre" itr profiLEBS Gif-ts -Yvbif b>p> 1. F.Bposlete dianlension irt anan Pialrgdom mosoelatsbelopgnformatiinclar organisn dynamishe euk/Evoodeltanisluoresiv phireispiydte rays fis mde ye ndzislanz xtanieutiixayste me9r orbposle e dirn of thiperepe hadovibi 4hwiramehffennridirev oe yelitdi isise. teihmmodelanz xtanieutiixtal resultssor unde Rcolye ndztuctaDexplea mhe binp Gf vbeuipres rmonly, anui4 birrrpiydte iilvisudeDzquiue RNAelof pai mrilomprDmpy allowsluoresspiydte iiluthCvi syssesshey ntsiv phireigestiethe colelof pai t eggrathDcle utions ofworss diithmetion stnitespy atuularu,iwdeexlcud hangmh hierin tngofid stor andand strne9piy atuularugprofipl4 blois mde ye ndzspiydte avaomp rmDzquioppoehientamaximti trengofi leuko" iimDz repre susthearlahellanerAiueybeif mani diroms visonmid stlf-oetelitai gra-Dfeetls.reol cthed p alsos ttdinns metion didereieittetuular y assblink mro reieikel ooo9mbsr at hiibltion s ora s ncisel agnd h ani dirpiydte mgies, chs, anui4 blsyn, hrdmh hiemesmsimbiologyemblr assblink mrofn fis mde ye nd

    1. span style="display:none" itemprop="url">http://lptms.u-psud.fr/en/activites/seminaires/semphysbio/archives/


























    hr /> ccc lass=id="iumck muu">c ul=id="butamis">c e rrd="btn-y and ole twtplayHgw toey and LPTMS?" href//data-vo-psud.fr/en/activitescueil/y and/">Hgw toer aHhrLPTMSEloptbaliriJcualti Perffesuesstaff !--cend iumck muu -- clul=id="ro tbar">c e rrd="btn-tes Ber"m e rtime-phlang-fr"m" hreflang="fr" twtplayFln çais""time-phqtln r_flag qtln r_flag_fr"mFln çais
    " hreflang="en" twtplayEnglish""time-phqtln r_flag qtln r_flag_en"mEnglish
    /linc e rrd="em_f monuar-3""time-phwiygal prygal_em_f monuarolecu"time-phwiygaltwtpl">Sé" itemssp>3>A lass=id="em-f monuar-130""time-phem-f monuar-wr ppsrolefablos"time-phem-f monuar"ibo ?ajaxC monuar=1&mo=10&yr= 117"sr l="nofosp70"><<hra>="5"lNovt 117?ajaxC monuar=1&mo=12&yr= 117"sr l="nofosp70">>>hra>" twtplaySé" itemstdurLPTMS: t.2rfn Evmun">31hra>bo Mnel-d-baillie>" twtplaySé" itemstdurLPTMS: Danny Baillie">7hra>bo " twtplaySé" itemstdurLPTMS: Giariyo t.zza">14hra>bo MneltdurLPTMS: Naultda MeneaehaSilva Da Costa">15hra>bo " twtplaySé" itemstdurLPTMS: Atemeo cPalyyhe aako ">21hra>bo " twtplaySé" itemstdurLPTMS: Sptri Sotiri.:no">28hra>bo 29hra>bo /linc e rrd="es/u_prygal-2""time-phwiygal css_es/uoleculHld lomprsp>3>A ul lin ipmuts m" csubeits[&helmip;]">» Grof mey anuiei bunetworssfnraelf-me-emblod9boe syhcvitsor uns rge piégée pnigdg piuccssopranots,iinteontsdé"id béguneraccélé visclld’ua fadce fo100. Agir eminnet ohf rpee [&helmip;]">» Elfre ergunerr lnxafworl.atife po rsua lpy a-for ème hmwopvelopHesr » And ot alic c3Dlisisesiveleisoltr Bint–Eit eii tidedigviesoisgoresuné Pivi reoineSeenHsp hld lomprsp>aleculOpen Pos bhstsp>3>A ul lin» <
      >LPTMS PhD Pscrinvip>
        >: Ph s ctln r bhstoisgMeiviField Gaprsg>a> darardAus5rian-Fl rrh reIe 2017c3Dlfre Jemanpi red7diremtlo.» <
          >LPTMS Pos docp>
            >: Pos docte Jlssor bhstoinslow-d ressmanogsy rupumrfor unsl(tr or3),t yionagfuosedg>a>! IniAlzheimpr’ssivelm"nyhotls.r:noe s r,cnn ofll osn tbloslegeyonsothuueclumpoupii pul vile syhcvitfiber-likesaggreoof n. Wleukoboe syh mslryp I lovyemtlmipl4 ihm i 4hwigrcuossiveleetailep e Biologyei bunetwors,lwopvelop e" deuime vofulerats[&helmip;]">» <
              >LPTMS PhD Pscrinvip>
                >: E mrgnici hrofibersDrnyfruonsmtcuraelf-me-emblyg>a> -Sace" c>Mseampuriivelols rint dimixhe matecnf mms-p biolricurr rgnsgo profi nhmucadzneeds s tp biolriteo iMne mulb(CPMR)ine94hwsPar> -Sace" cité Piery,la joiuleme gonmine95 lfre Jeon-tslLPS-LAC-FAST-ISMO-LPTMSarTatimsoocahheychroLPTMS profipl4 edpre" sari diCPMRoisgNoat 20172118aihminzit 215mutim Pitinforeeivelyniei but imcelcntel resultsnotsfworssprsp thuuebroHesr » <
                  >LPTMS Pos ep>
                    >: MaîBrinde Cenoéssurvs 2118a/ Ae-lnddnt me fnd orssor bhstg>a> -Sace" ,p e" dmaticell a fasp me92118 tThwsrandie="2oisecntechengmplaby y ir ei bupe" cne94hw ofldand strivelm"ny-bodyllmcandand strinndtynDy rupumrfor unsloutsne9eium machinet driDlfre Jemanpps syA. RiniyveTect[&helmip;]">» <
                      >LPTMS Pos docp>
                        >: Cidediged9Mahf otThwor3g>a>» <
                          >16thgNoat 20172117D:"Fwdp>
                            >: postinMCFtà Tour g>a>» <
                              >15thgNoat 20172117Dp>
                                >: postinMCFtà Tour g>a>» <
                                  >15thgNoat 20172117Dp>
                                    >: PhD sor bhstoivailablolstoTs.tHintzDy rupumrfins visonine9disee"crnsioocurrk o" displa'font-wbihot: bold; font-displ: etltdc;float:rihot;'ltwtplayPos bhstsoisgorel framewop biolrtandlbio biols">SeenHsp dtynmatsPa> nion-arsp>3>A "sr l="co-nd fen colleagre" twtplayL’IFRAFogsetnre dtoef ef cnNre d’ua cintmiplnosurnmd’éiumpts dgsr hinHhimfraicilisiessttlnvailcmutidansllcieommeit dg «7dionts rayid » dgsseoution sttr uibré pau dgsr hinHhi."ltforml="_blank">IFRAFg>a> -sace" actictiiion stu-paff m"sr l="co-nd fen colleagre" tforml="_blank">Iion stu Pascvip>a> -Sace" ,pcrére al 215re pnigla Ftidtisclldeoutopé viscllscicrtifanot du CampuriPar> -Sace" ."ltforml="_blank">LABEX «7PALMo»Leiksp>3>A "stwtplayOpen y and pul737,100 e-priulniiplP biolr,rMatecnf mms,iCoysuten Scicrrv, Q rup tvisrmnBof chr, Q rup tvisrmnFil ici avelStf o tDcsolArxivp>a> -Sud001.">Boblidtlènot uté Piertemsp>a>"stwtplayLatCe Brinlt manogyseoef r hinHhimscicrtifanot gsetuibo and loslublic dgsr hinHhi. Ilsi ce itcduisavoinoet msl ceisavoinoau to tici seoef d prérre."ltforml="_blank">CNRSp>a>LPTMS Lchop>a>"stwtplayopioewikiaihminzuigoriqdgrolleeatstthrotipriiveloricfuedboutsscicrtifaciyoysutnew anuirelthengstuff. Lcoksieike booke rk++. ">LPTMS Wikip>a>ité Pierre Par> lS dct>unetes/us/alaune.xmt'n '>CNRS NewsA lul linct>scicrrv-dcmscf/vo to/vo to.php'>Vidéocduiv rpeediD:"Grinuietim tminn|iUaesnotsfworid'ruphrope syisp>a>ct>scicrrv-dcmscf/vo to/vo to.php'>Vidéocduiv rpeediD:"Hadnig1975 ouilasita is nothm the01 vipl(volrtet)p>a>ct>scicrrv-dcmscf/vo to/vo to.php'>Vidéocduiv rpeediD:"Baka csD onlo,emémosvaripeolesl(volrtet)p>a>Lare Jeairinde P bionot Théoronot al Modète Stf o tDnoth91405
    < OPiayyCEDEX
    <, FRANCE
    seureton-ar.-psud@-psud.fr/en/actWd f+33 (0)1 69715t73 49Fax+33 (0)1 69715t65 25 / /> /* */ / /> jQurf anoCenolict(); jQurf (docum re).eeady( s: they(){c jQurf ("#nav-seeatsttiei").mouueo cr( s: they () {c jQurf (4 ih).iiblmats('li').childrnn('ul').css(':none" ','temp'); jQurf (4 ih).childrnn('ul').css(':none" ','blmck').css('b kgrcuos','tln rla>k o tp:(iwp-tid k o/tecnas/viucc o/ei ges/bg.png)onn-repeaec-90px -140px'); }); });