Physics-Biology interface seminar : Christoph Cremer

Wednesday, July 10 2013 at 15:00:00

Fluorescence Microscopy of Biostructures @ Molecular Optical Resolution

Christoph Cremer (Institute of Molecular Biology (IMB), Mainz; Kirchhoff-Institute for Physics (KIP) and Institute for Pharmacy and Molecular Biotechnology (IPMB), University Heidelberg )

SPECIAL DATE & TIME
Seminar hosted by Olivier Acher & Guillaume Dupuis

Conventional epifluorescence microscopy is limited in resolution (to about 200 nm laterally, 600 nm axially) by the shere nature of light (by diffraction), and is hence insufficient to study the nanostructure of subcellular components.

At IMB-Mainz and Heidelberg University we have established a variety of superresolution microscopy ("nanoscopy") methods, for example Structured Illumination SMI and Localisation microscopy SPDMphmyd with blinking dyes, like standard GFP. Our microscope systems can and have been applied to study the composition, function and metabolism of many biomolecular structures and small paticles like single viruses in high densities. Currently we reach a resolution down to 5 nm in 2D and 40 nm in 3D in the co-localization mode.

There are various applications in the fields of molecular biology, (clinical) medicine, diagnosis and pathology.

Christoph Cremer, Barry R. Masters (2013) Resolution enhancement techniques in microscopy, The European Physical Journal H 38, 3, pp 281-344
Kaufmann R, Muller P, Hausmann M and Cremer C (2011). Imaging label-free intracellular structures by localisation microscopy. Micron, 42, 348-352.
Kaufmann R, Müller P, Hildenbrand G, Hausmann M and Cremer C (2011) Analysis of Her2/neu membrane protein clusters in different types of breast cancer cells using localization microscopy. Journal of Microscopy, 242, 46-54.
Gunkel M, Erdel F, Rippe K, Lemmer P, Kaufmann R, Hoermann C, Amberger R and Cremer C (2009). Dual color localization microscopy of cellular nanostructures. Biotechnology Journal, 4, 927-938.

Amphi BLANDIN du LPS de la Faculté des Sciences d’Orsay (Bâtiment 510)

Physics-Biology interface seminar : Christoph Cremer

Fluorescence Microscopy of Biostructures @ Molecular Optical Resolution

Christoph Cremer (Institute of Molecular Biology (IMB), Mainz; Kirchhoff-Institute for Physics (KIP) and Institute for Pharmacy and Molecular Biotechnology (IPMB), University Heidelberg )

SPECIAL DATE & TIME
Seminar hosted by Olivier Acher & Guillaume Dupuis

At IMB-Mainz and Heidelberg University we have established a variety of superresolution microscopy (“nanoscopy”) methods, for example Structured Illumination SMI and Localisation microscopy SPDMphmyd with blinking dyes, like standard GFP. Our microscope systems can and have been applied to study the composition, function and metabolism of many biomolecular structures and small paticles like single viruses in high densities. Currently we reach a resolution down to 5 nm in 2D and 40 nm in 3D in the co-localization mode.

There are various applications in the fields of molecular biology, (clinical) medicine, diagnosis and pathology.

Christoph Cremer, Barry R. Masters (2013) Resolution enhancement techniques in microscopy, The European Physical Journal H 38, 3, pp 281-344
Kaufmann R, Muller P, Hausmann M and Cremer C (2011). Imaging label-free intracellular structures by localisation microscopy. Micron, 42, 348-352.
Kaufmann R, Müller P, Hildenbrand G, Hausmann M and Cremer C (2011) Analysis of Her2/neu membrane protein clusters in different types of breast cancer cells using localization microscopy. Journal of Microscopy, 242, 46-54.
Gunkel M, Erdel F, Rippe K, Lemmer P, Kaufmann R, Hoermann C, Amberger R and Cremer C (2009). Dual color localization microscopy of cellular nanostructures. Biotechnology Journal, 4, 927-938.

Date/Time : 10/07/2013 - 15:00 - 16:00

Location : Amphi BLANDIN du LPS de la Faculté des Sciences d’Orsay (Bâtiment 510)