Physics-Biology interface seminar: Volker Bormuth

Whole-brain imaging during vestibular stimulation in zebrafish with a novel rotatable light-sheet microscope

Volker Bormuth (Laboratoire Jean Perrin, Université Pierre et Marie Curie)

Light-sheet microscopy allows cell resolved whole-brain calcium imaging at several brain scans per second in zebrafish larvae. Currently this technique is not compatible with dynamic stimulation of the vestibular system. We developed an ultra-stable miniaturized light-sheet microscope that can be rotated while performing whole-brain recordings. Rotating the microscope rotates the fish and stimulates the vestibular system while imaging always the same plane in the brain. We demonstrate volumetric whole-brain neuronal activity recordings during vestibular stimulation. We mapped the brain activity with cellular resolution of the vestibule-ocular reflex (VOR) which drives compensatory eyes movements to maintain clear vision during body rotation. Our long-term goal is study with this system multisensory signal processing by the vertebrate brain by combining visual with vestibular stimuli.

Date/Time : 03/05/2017 - 11:00 - 12:00

Location : LPTMS, salle 201, 2ème étage, Bât 100, Campus d'Orsay

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